PCR/RT- PCR in situ PDF Download. Download free ebook of PCR/RT- PCR in situ in PDF format or read online by Gerard Morel,Mireille Raccurt 9780849300417 Published on 2002-09-27 by CRC Press
Although the polymerase chain reaction has revolutionized genetic analysis by amplifying rare nucleic acid sequences, the in situ application is the only method that allows the localization of amplified signal within tissue structure. The applications of in situ polymerase chain reaction have greatly enhanced the field of investigation in many disciplines, including viral infections, gene modification, tumor diagnosis, gene therapy, and cellular distribution of rare mRNA copies. PCR/RT-PCR in situ: Light and Electron Microscopy covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and RNA in tissues and cell cultures at the light and electron microscopy levels. Written by experts in this field, the book provides theoretical consideration, as well as practical approaches to in situ PCR. The authors provide detailed protocols for each step, including the preparation of tissue samples, the rationale for the design of primers and revelation. They also emphasize the need for appropriate controls to meet the requirements of in situ PCR and RT-PCR specificity. Organized in a user-friendly two-column format, this book will provide you with tools necessary to perform and optimize these sensitive and powerful techniques in your research protocols.
This Book was ranked at 15 by Google Books for keyword Electron Microscopy.
Book ID of PCR/RT- PCR in situ's Books is PvPjnQEACAAJ, Book which was written by Gerard Morel,Mireille Raccurt have ETAG "/G0lCt8fI/8"
Book which was published by CRC Press since 2002-09-27 have ISBNs, ISBN 13 Code is 9780849300417 and ISBN 10 Code is 084930041X
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